| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ono, Hako (11)
|
MRBAD |
32.12 |
32.48 |
0.36 |
| 2 |
McGlynn, Elena M (11)
|
MRBAD |
32.19 |
32.56 |
0.37 |
| 3 |
Turnbull, Hayden R (12)
|
MRBAD |
37.22 |
32.71 |
-4.51 |
| 4 |
Tait, Charlotte J (11)
|
CTGRYM |
37.90 |
36.80 |
-1.10 |
| 5 |
Krogsgaard, Linnea A (12)
|
MREAST |
39.91 |
36.98 |
-2.93 |
| 6 |
Chang, Barbara A (12)
|
MREAST |
35.95 |
37.05 |
1.10 |
| 7 |
Garcia-Alonso, Alexia (11)
|
CTGRYM |
44.66 |
37.34 |
-7.32 |
| 8 |
O'Donnell, Keira T (11)
|
CTGRYM |
40.16 |
37.63 |
-2.53 |
| 9 |
Kim, Cameron E (11)
|
MREAST |
42.12 |
42.51 |
0.39 |
| 10 |
Bloom, Zadie R (12)
|
MREAST |
45.26 |
43.02 |
-2.24 |
| 11 |
Faulkner, Sofia R (11)
|
MREAST |
49.86 |
43.88 |
-5.98 |
| 12 |
O'Rourke, Ann M (11)
|
MREAST |
44.11 |
44.12 |
0.01 |
| 13 |
Wepman, Finley B (11)
|
MREAST |
43.70 |
46.97 |
3.27 |
| 14 |
Hong, Lauren (12)
|
CTGRYM |
44.29 |
47.22 |
2.93 |
|
Kapoor, Keisha (11)
|
MREAST |
|
DQ |
- |