| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sandoval, Joselyn (14)
|
MRRED |
29.02 |
28.23 |
-0.79 |
| 2 |
Heerey, Karolina A (14)
|
MRCIY |
28.51 |
28.94 |
0.43 |
| 2 |
Huang, Isabell (13)
|
MRFLY |
29.35 |
28.94 |
-0.41 |
| 4 |
McKinney, Emilia W (14)
|
MRCIY |
29.91 |
30.29 |
0.38 |
| 5 |
Trinidad, Chelanny A (13)
|
MRRED |
30.44 |
30.34 |
-0.10 |
| 6 |
Han, Marianne H (13)
|
MRFLY |
30.21 |
30.63 |
0.42 |
| 7 |
Amankwa-Jones, Isabella B (14)
|
MRRED |
30.79 |
30.93 |
0.14 |
| 8 |
McNally, Jessica J (13)
|
MRCIY |
30.98 |
31.12 |
0.14 |
| 9 |
Roder, Gwyneth J (13)
|
MRCIY |
32.70 |
31.45 |
-1.25 |
| 10 |
Kohm, Sienna C (14)
|
MRCIY |
29.74 |
31.99 |
2.25 |
| 11 |
Menard, Madelyn X (13)
|
MRCIY |
32.52 |
32.62 |
0.10 |
| 12 |
Oei, Jolene G (13)
|
MRFLY |
33.90 |
33.75 |
-0.15 |
| 13 |
Lim, Kaylie (13)
|
MRFLY |
33.96 |
34.86 |
0.90 |