| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Han, Marianne H (13)
|
MRFLY |
33.39 |
33.04 |
-0.35 |
| 2 |
McKinney, Emilia W (14)
|
MRCIY |
43.85 |
33.76 |
-10.09 |
| 3 |
Sandoval, Joselyn (14)
|
MRRED |
34.91 |
33.94 |
-0.97 |
| 4 |
Kohm, Sienna C (14)
|
MRCIY |
33.37 |
35.09 |
1.72 |
| 5 |
Trinidad, Chelanny A (13)
|
MRRED |
38.00 |
35.62 |
-2.38 |
| 6 |
Heerey, Karolina A (14)
|
MRCIY |
|
35.67 |
- |
| 7 |
McNally, Jessica J (13)
|
MRCIY |
36.56 |
36.42 |
-0.14 |
| 8 |
Huang, Isabell (13)
|
MRFLY |
36.64 |
36.56 |
-0.08 |
| 9 |
Amankwa-Jones, Isabella B (14)
|
MRRED |
37.52 |
37.23 |
-0.29 |
| 10 |
Menard, Madelyn X (13)
|
MRCIY |
43.88 |
37.76 |
-6.12 |
| 11 |
Oei, Jolene G (13)
|
MRFLY |
48.33 |
40.58 |
-7.75 |
| 12 |
Lim, Kaylie (13)
|
MRFLY |
|
43.70 |
- |