| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shnayderman, Alisa (13)
|
MRMPNY |
27.79 |
27.15 |
-0.64 |
| 2 |
Vodopyanov, Elizabeth (13)
|
MRSIAC |
28.26 |
28.13 |
-0.13 |
| 3 |
Roudnitsky, Julia (14)
|
MRFREE |
28.82 |
29.60 |
0.78 |
| 4 |
Attia, Kinzie M (13)
|
MRSIAC |
31.40 |
30.46 |
-0.94 |
| 5 |
Lee, Kaitlyn (13)
|
MRSIAC |
30.23 |
30.75 |
0.52 |
| 6 |
Schroeder, Emily (14)
|
MRSIAC |
31.03 |
30.83 |
-0.20 |
| 7 |
Paventi, Ashley (14)
|
MRSIAC |
31.33 |
31.61 |
0.28 |
| 8 |
Troncoso, Emma H (13)
|
MRFREE |
32.45 |
32.54 |
0.09 |
| 9 |
Paventi, Lauren (14)
|
MRSIAC |
31.64 |
32.57 |
0.93 |
| 10 |
Poliakova, Vladislava (13)
|
MRFREE |
35.35 |
32.96 |
-2.39 |
| 11 |
Nathanson, Michelle (13)
|
MRFREE |
33.51 |
33.26 |
-0.25 |
| 12 |
Torres, Amaliah J (13)
|
MRFREE |
39.66 |
37.09 |
-2.57 |