| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Knuth, Elisabeth (12)
|
MRCFJV |
36.57 |
36.53 |
-0.04 |
| 2 |
Kuzicki, Isabella R (11)
|
MRYBAR |
42.98 |
39.11 |
-3.87 |
| 3 |
Ramos, Aliza D (12)
|
MRRFAC |
37.30 |
39.49 |
2.19 |
| 4 |
Humphrey, Lyla R (12)
|
MRPBAC |
44.90 |
40.47 |
-4.43 |
| 5 |
Jones, Athena S (12)
|
MRNBS |
42.19 |
40.92 |
-1.27 |
| 6 |
Furfaro, Kira J (12)
|
MRCFJV |
40.75 |
40.94 |
0.19 |
| 7 |
Kurth, Katie L (12)
|
MRCFJV |
40.00 |
41.65 |
1.65 |
| 8 |
Baideme, Natalie B (11)
|
MRWSSC |
42.46 |
41.79 |
-0.67 |
| 9 |
Gillman, Helen M (12)
|
MRWSSC |
42.26 |
42.12 |
-0.14 |
| 10 |
Broge, Eleanor H (11)
|
MRWSSC |
43.34 |
42.94 |
-0.40 |
| 11 |
Baker, Emily S (12)
|
MRNDAC |
47.53 |
45.82 |
-1.71 |
| 12 |
Ingenito, MariaStella * (12)
|
MRRFAC |
47.28 |
46.24 |
-1.04 |
| 13 |
Blanco, Isabella L (11)
|
MRNBS |
47.11 |
46.52 |
-0.59 |
| 14 |
Costello, Treven M (12)
|
MRYBAR |
50.19 |
46.96 |
-3.23 |
| 15 |
Gillman, Katherine V (11)
|
MRWSSC |
49.15 |
47.53 |
-1.62 |
| 16 |
Horan, Skylar G (11)
|
MRVAC |
51.52 |
50.33 |
-1.19 |
| 17 |
Chiappetta, Isabella L (12)
|
MRRFAC |
49.82 |
51.50 |
1.68 |
| 18 |
Maddu, Aanya H (12)
|
MRMWDA |
53.32 |
53.12 |
-0.20 |
| 19 |
Mejia, Annabelle L (11)
|
MRNBS |
|
1:08.56 |
- |
| 20 |
Ramaglia, Autumn C (12)
|
MRNBS |
|
1:11.89 |
- |
| 21 |
Gillman, Elizabeth M (12)
|
MRWSSC |
|
1:31.62 |
- |
|
Luna, Isabella J (11)
|
MRNBS |
|
DQ |
- |
|
Laufman, Alyza J (11)
|
MRCFJV |
47.69 |
NS |
- |