| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dixon, Grayson B (13)
|
MRBGNW |
55.71 |
55.27 |
-0.44 |
| 2 |
Darder, Sean C (13)
|
MRPAC |
56.57 |
55.29 |
-1.28 |
| 3 |
Mummery, Conor J (13)
|
MRNBS |
56.02 |
55.45 |
-0.57 |
| 4 |
Acocella, Andrew C (13)
|
MRBGNW |
56.67 |
56.11 |
-0.56 |
| 5 |
Espinoza-Soriano, Jorge (13)
|
MRMHHW |
58.36 |
56.48 |
-1.88 |
| 6 |
Siminerio, Nate M (13)
|
MRBGNW |
57.42 |
57.10 |
-0.32 |
| 7 |
Molloy, Mark P (13)
|
MRCOND |
58.34 |
57.18 |
-1.16 |
| 8 |
Ruffy, Rhyley X (13)
|
MRNYS |
59.07 |
57.27 |
-1.80 |
| 9 |
Felker, Christopher D (13)
|
MRMWDA |
58.40 |
57.39 |
-1.01 |
| 10 |
Kim, Elijah S (13)
|
MRPAC |
59.22 |
58.71 |
-0.51 |
| 11 |
Berko, Reuben L (13)
|
MRNDAC |
59.05 |
58.91 |
-0.14 |
|
Sevilla, William H (13)
|
MREAST |
57.99 |
1:00.24 |
2.25 |