| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jia, Nicole X (12)
|
MRMPNY |
32.06 |
31.66 |
-0.40 |
| 1 |
Masella, Catherine R (12)
|
MRYFD |
35.65 |
36.40 |
0.75 |
| 2 |
Curinnaia, Elena (12)
|
MRMPNY |
34.29 |
31.67 |
-2.62 |
| 2 |
McLoughlin, McKayla E (12)
|
MRBAD |
35.74 |
37.23 |
1.49 |
| 3 |
Turnbull, Hayden R (12)
|
MRBAD |
32.30 |
32.29 |
-0.01 |
| 4 |
Suharev, Anna M (12)
|
MRFREE |
32.04 |
32.68 |
0.64 |
| 5 |
Alexander, Ayla S (12)
|
MRLGAC |
32.39 |
32.93 |
0.54 |
| 6 |
Fasolino, Sofia (12)
|
MRWEST |
33.87 |
33.57 |
-0.30 |
| 7 |
Canales Alvizuri, Isabella N (12)
|
MRLIE |
33.91 |
33.62 |
-0.29 |
| 8 |
Cheung, Isabella (12)
|
MRCENT |
34.96 |
33.77 |
-1.19 |
| 9 |
Galimi, Alexa A (12)
|
MRBAD |
34.70 |
33.91 |
-0.79 |
| 10 |
McFarland, Brynn C (12)
|
MRBAD |
34.57 |
34.05 |
-0.52 |
| 11 |
Daley, Isabelle A (12)
|
MRCENT |
34.78 |
34.18 |
-0.60 |
| 12 |
DiStefano, Ella (12)
|
MRTRI |
34.25 |
34.23 |
-0.02 |
| 13 |
Schaffer, Alexis B (12)
|
MRWEST |
35.78 |
34.81 |
-0.97 |
| 14 |
Meyer, Suraya B (12)
|
MRYFD |
34.59 |
34.90 |
0.31 |
| 15 |
Sherman, Alexa L (12)
|
MRLIE |
32.64 |
34.96 |
2.32 |
| 16 |
Palmieri, Beatrice N (12)
|
MRTRI |
35.75 |
34.97 |
-0.78 |
| 17 |
Yu, Charis (12)
|
MRMPNY |
34.57 |
35.14 |
0.57 |
| 18 |
Leal, Jazmine (12)
|
MRRED |
35.56 |
35.35 |
-0.21 |