| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bronskikh, Valeriia N (12)
|
MRFREE |
27.93 |
27.50 |
-0.43 |
| 1 |
Jia, Nicole X (12)
|
MRMPNY |
28.35 |
28.22 |
-0.13 |
| 2 |
Canales Alvizuri, Isabella N (12)
|
MRLIE |
28.19 |
27.60 |
-0.59 |
| 2 |
Gourevitch, Olivia K (12)
|
MRBAD |
28.65 |
28.24 |
-0.41 |
| 3 |
Cheung, Isabella (12)
|
MRCENT |
29.23 |
28.98 |
-0.25 |
| 3 |
Beleffi, Bianca (12)
|
MRLGAC |
30.30 |
31.12 |
0.82 |
| 4 |
Leal, Jazmine (12)
|
MRRED |
28.72 |
29.04 |
0.32 |
| 4 |
Meyer, Suraya B (12)
|
MRYFD |
29.96 |
31.43 |
1.47 |
| 5 |
Sherman, Alexa L (12)
|
MRLIE |
28.17 |
29.33 |
1.16 |
| 5 |
Masella, Catherine R (12)
|
MRYFD |
30.73 |
31.54 |
0.81 |
| 6 |
McLoughlin, McKayla E (12)
|
MRBAD |
29.52 |
29.53 |
0.01 |
| 7 |
Wong, Madeleine C (12)
|
MRFLY |
28.83 |
29.73 |
0.90 |
| 8 |
McFarland, Brynn C (12)
|
MRBAD |
29.60 |
29.89 |
0.29 |
| 9 |
Daley, Isabelle A (12)
|
MRCENT |
29.39 |
29.93 |
0.54 |
| 10 |
Tse, Avery (12)
|
MRMAKO |
29.92 |
30.37 |
0.45 |
|
Boehm, Jacqueline R (12)
|
MRQNS |
30.29 |
NS |
- |