| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Albra, Tristan E (12)
|
MRHVD |
28.47 |
27.63 |
-0.84 |
| 2 |
Johnson, Ethan O (12)
|
MRVAC |
28.59 |
28.61 |
0.02 |
| 3 |
Martin, Daniel (12)
|
MRNBS |
29.07 |
28.65 |
-0.42 |
| 4 |
Rettig, Adam (12)
|
MRPAC |
29.32 |
28.70 |
-0.62 |
| 5 |
Bucko, Cameron L (12)
|
MRPAC |
29.60 |
28.80 |
-0.80 |
| 6 |
Milner, Adam J (12)
|
MRPAC |
28.74 |
28.84 |
0.10 |
| 7 |
Vybihal, Adam J (12)
|
MRYBAR |
28.85 |
28.93 |
0.08 |
| 8 |
Kim, Gianluca G (12)
|
MRPAC |
28.53 |
28.94 |
0.41 |
| 9 |
Salomatov, Maxim A (12)
|
MRBGNW |
29.47 |
29.20 |
-0.27 |
| 10 |
Silverman, Aidan J (12)
|
MRCOND |
28.76 |
29.26 |
0.50 |
| 11 |
Lazarski, Jacob B (12)
|
MRNBS |
30.30 |
29.35 |
-0.95 |
| 12 |
Biagi, Maximillian R (12)
|
MRCFJV |
29.52 |
29.75 |
0.23 |
| 13 |
Kavanagh, Camryn P (12)
|
MRNBS |
30.34 |
30.01 |
-0.33 |
| 14 |
John, Matthew R (12)
|
MRCOND |
30.18 |
30.05 |
-0.13 |
| 15 |
Zhang, Stanley (12)
|
MRMWDA |
30.49 |
30.18 |
-0.31 |
| 16 |
Bagriyanik, Sarp A (12)
|
MRPATS |
29.95 |
30.33 |
0.38 |
| 17 |
Brockmann, Alexander J (12)
|
MRNYS |
30.44 |
30.62 |
0.18 |