| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Monnelly, Mikaela N (12)
|
MRBGNW |
31.29 |
30.34 |
-0.95 |
| 2 |
Salomatov, Anna A (12)
|
MRBGNW |
31.09 |
30.92 |
-0.17 |
| 3 |
Oh, Sophia C (12)
|
MRBGNW |
30.32 |
31.20 |
0.88 |
| 3 |
Sitner, Estella R (12)
|
MRCOND |
33.46 |
31.20 |
-2.26 |
| 5 |
Fernstrom, Abigail R (12)
|
MRBGNW |
32.59 |
31.88 |
-0.71 |
| 6 |
Joachim, Gianna M (12)
|
MRNBS |
31.32 |
31.97 |
0.65 |
| 7 |
Abulencia, Annelise M (12)
|
MRCFJV |
31.35 |
32.14 |
0.79 |
| 8 |
Edelmann, Paige (12)
|
MRCFJV |
32.10 |
32.64 |
0.54 |
| 9 |
Wallace, Lia O (12)
|
MRRA |
32.62 |
32.94 |
0.32 |
| 11 |
Eckert, Lucy M (12)
|
MRNDAC |
32.75 |
33.35 |
0.60 |
| 12 |
Pfluger, Allison E (12)
|
MRBGNW |
33.74 |
33.57 |
-0.17 |
| 13 |
Broge, Amelia H (12)
|
MRWSSC |
33.73 |
33.77 |
0.04 |
| 14 |
Forger, Chloe A (12)
|
MRSMC |
33.91 |
34.03 |
0.12 |
| 15 |
Laufman, Alyza J (12)
|
MRCFJV |
33.25 |
34.07 |
0.82 |
| 16 |
Jensen, Remi M (12)
|
MRPAC |
34.38 |
34.31 |
-0.07 |