| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eckert, Lucy M (11)
|
MRNDAC |
36.25 |
36.63 |
0.38 |
| 2 |
Nitzkorski, Ella F (12)
|
MRRFAC |
36.82 |
36.90 |
0.08 |
| 3 |
Yereance, Addison L (11)
|
MRRFAC |
38.35 |
37.89 |
-0.46 |
| 4 |
Catenacci, Emma E (12)
|
MRNDAC |
43.41 |
38.70 |
-4.71 |
| 5 |
Courtney, Charlotte T (11)
|
MRNDAC |
45.55 |
39.87 |
-5.68 |
| 6 |
Lopez-leon, Camila V (12)
|
MRNDAC |
46.33 |
46.40 |
0.07 |
| 7 |
Miller, Adelyn J (12)
|
NESNAP |
55.36 |
46.76 |
-8.60 |
| 8 |
FitzgeraldVanloan, Hadley (12)
|
MRNDAC |
58.18 |
47.69 |
-10.49 |
| 9 |
Miller, Braelyn G (12)
|
NESNAP |
45.99 |
48.50 |
2.51 |
| 10 |
Petrocelli, Annabella S (11)
|
MRRFAC |
|
1:00.83 |
- |
|
Kui, Hannah F (11)
|
MRRFAC |
|
DQ |
- |
|
Kaiser, Norah F (12)
|
MRNDAC |
40.35 |
NS |
- |