| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Humphrey, Ella M (14)
|
MRPBAC |
27.53 |
27.34 |
-0.19 |
| 2 |
Briceno, Isabella (13)
|
MRNYS |
26.91 |
27.80 |
0.89 |
| 3 |
White, Emily G (13)
|
MRNYS |
27.62 |
28.01 |
0.39 |
| 4 |
Zellner-Portela, Natasha N (13)
|
MRNYS |
27.40 |
28.41 |
1.01 |
| 5 |
Piscitella, Vivian I (14)
|
MRNYS |
28.24 |
28.64 |
0.40 |
| 6 |
McAuley, Nadia A (14)
|
MRNYS |
29.11 |
28.72 |
-0.39 |
| 7 |
Nedorost, Paulette (14)
|
MRVAC |
28.04 |
28.79 |
0.75 |
| 8 |
Messing, Samantha K (13)
|
MRVAC |
28.56 |
28.84 |
0.28 |
| 9 |
Thompson, Samantha K (13)
|
MRVAC |
28.08 |
28.86 |
0.78 |
| 10 |
Fogarty, Ireland C (13)
|
MRVAC |
31.74 |
31.41 |
-0.33 |
| 11 |
Ruiz, Beverly C (14)
|
MRMHHW |
33.00 |
31.90 |
-1.10 |
| 12 |
Louey, Sarah E (14)
|
MRVAC |
|
34.36 |
- |
| 13 |
Aguilar Perez, Vanessa (14)
|
MRMHHW |
|
34.73 |
- |
| 14 |
Nolan, Makenzie A (14)
|
MRVAC |
|
34.93 |
- |