| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Benner, Cassandra J (9)
|
ADHAWK |
52.16 |
46.76 |
-5.40 |
| 2 |
Fiske, Kelsey A (10)
|
MRHVD |
48.75 |
47.18 |
-1.57 |
| 3 |
Kenney, Sage S (9)
|
ADHAWK |
1:01.41 |
49.52 |
-11.89 |
| 4 |
Schmitt, Penelope A (10)
|
MRRFAC |
47.40 |
50.02 |
2.62 |
| 5 |
Zurita, Brittany (10)
|
MRMHHW |
54.91 |
52.90 |
-2.01 |
| 6 |
DiBenedetto, Milania A (9)
|
MRPATS |
|
54.20 |
- |
| 7 |
Virmani, Samaya K (9)
|
CTOAK |
57.14 |
56.99 |
-0.15 |
| 8 |
McKenna, Colette (9)
|
MRMHHW |
|
58.71 |
- |
| 9 |
LaVallee, Paige (9)
|
MRMWDA |
|
1:04.22 |
- |
|
Drivas, Margaret B (10)
|
ADHAWK |
|
DQ |
- |
|
Planthaber, Isabel J (10)
|
ADHAWK |
|
DQ |
- |