| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mummery, Conor J (14)
|
MRNBS |
25.11 |
25.29 |
0.18 |
| 2 |
Espinoza-Soriano, Jorge (13)
|
MRMHHW |
25.56 |
25.92 |
0.36 |
| 3 |
Berko, Reuben L (13)
|
MRNDAC |
25.97 |
26.49 |
0.52 |
| 4 |
Dutta, Abir A (14)
|
CTOAK |
26.86 |
26.96 |
0.10 |
| 5 |
Lynaugh, Matthew L (13)
|
MRMWDA |
28.59 |
27.52 |
-1.07 |
| 6 |
Sheeley, Bennett A (13)
|
ADHAWK |
29.98 |
28.32 |
-1.66 |
| 7 |
Touijar, Ali (14)
|
MRMHHW |
29.49 |
29.06 |
-0.43 |
| 8 |
LoPresti, Pietro R (14)
|
MRNBS |
28.78 |
29.81 |
1.03 |
| 9 |
Howard, Sam W (14)
|
MRRFAC |
30.09 |
30.09 |
0.00 |
| 10 |
Strober, Cale S (14)
|
CTOAK |
29.02 |
30.33 |
1.31 |
| 11 |
Raszkiewicz, Alex R (13)
|
MRRFAC |
31.38 |
31.33 |
-0.05 |
| 12 |
Bodo, Brayden M (13)
|
MRHVD |
32.33 |
31.62 |
-0.71 |
| 13 |
Martinez, Eztli (13)
|
MRMHHW |
37.67 |
32.08 |
-5.59 |
| 14 |
Herrera, Alejandro (13)
|
MRPATS |
33.70 |
32.53 |
-1.17 |
| 15 |
Kui, Adam W (14)
|
MRRFAC |
33.05 |
32.89 |
-0.16 |
| 16 |
Shaw, Jaden K (14)
|
MRMWDA |
33.79 |
35.00 |
1.21 |
| 17 |
Murray, Sean M (13)
|
MRNDAC |
36.59 |
39.01 |
2.42 |
| 18 |
Chiappetta, Paolo A (13)
|
MRRFAC |
40.14 |
40.58 |
0.44 |
| 19 |
Eichenhofer, George H (13)
|
MRHVD |
44.96 |
40.90 |
-4.06 |