| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sarpong, Afia (17)
|
MRHVD |
24.79 |
25.92 |
1.13 |
| 2 |
Skerritt, Katherine C (15)
|
MRHVD |
25.77 |
26.18 |
0.41 |
| 3 |
Clark, Campbell U (17)
|
MRRFAC |
25.91 |
26.41 |
0.50 |
| 4 |
Tirch, Doriana R (15)
|
MRHVD |
26.39 |
27.40 |
1.01 |
| 5 |
Johnson, Jasmine K (17)
|
CTOAK |
26.11 |
27.70 |
1.59 |
| 6 |
Strober, Lila N (17)
|
CTOAK |
26.07 |
27.75 |
1.68 |
| 7 |
Napoli, Julia R (17)
|
ADHAWK |
25.90 |
27.81 |
1.91 |
| 8 |
Kostelni, Christina T (15)
|
CTOAK |
27.23 |
28.10 |
0.87 |
| 9 |
Seldin, Emily (17)
|
MRRFAC |
27.79 |
28.75 |
0.96 |
| 10 |
Casucci, Kira E (15)
|
MRHVD |
28.14 |
29.06 |
0.92 |
| 11 |
Thomas, Mikayla M (17)
|
MRPATS |
28.27 |
29.61 |
1.34 |
| 12 |
Wallace, Paige A (15)
|
CTOAK |
28.48 |
29.68 |
1.20 |
| 13 |
Wiltshire, Saniyah C (16)
|
MRPATS |
28.22 |
29.78 |
1.56 |
| 14 |
Odums, Kaelynn (15)
|
MRMHHW |
29.78 |
30.00 |
0.22 |
| 15 |
Wilson, Emma M (16)
|
MRRFAC |
27.56 |
30.25 |
2.69 |
| 16 |
Espinoza-Soriano, Lidia A (15)
|
MRMHHW |
32.09 |
34.28 |
2.19 |
| 17 |
Perez-Lopez, Yana (16)
|
MRMHHW |
36.02 |
35.74 |
-0.28 |
| 18 |
Touijar, Heba (15)
|
MRMHHW |
34.49 |
35.79 |
1.30 |
| 19 |
Perez-Lopez, Linsay (17)
|
MRMHHW |
34.84 |
36.70 |
1.86 |
| 20 |
Marmolejos, Krystell (16)
|
MRHVD |
|
47.00 |
- |
|
Samko, Clara M (16)
|
ADHAWK |
24.63 |
DQ |
- |
|
Horgan, Rosemary E (17)
|
MRHVD |
26.32 |
NS |
- |
|
Hannon, Morgan A (16)
|
MRHVD |
25.82 |
NS |
- |
|
Lopez, Idalaih (17)
|
MRMHHW |
40.61 |
NS |
- |