| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fagundes, Sofia (11)
|
MRHAUP |
40.58 |
40.12 |
-0.46 |
| 2 |
O'Shea, Avienne (11)
|
MREHY |
39.37 |
40.67 |
1.30 |
| 3 |
Acosta, Veronica (12)
|
MRHAUP |
44.78 |
41.59 |
-3.19 |
| 4 |
Grande, Molly (12)
|
MREHY |
44.11 |
42.15 |
-1.96 |
| 5 |
Flaherty, Reagan E (11)
|
MRCSDC |
43.41 |
42.79 |
-0.62 |
| 6 |
John, Leah M (12)
|
MRSSC |
45.91 |
43.63 |
-2.28 |
| 7 |
Cox, Eva F (11)
|
MRHYB |
42.60 |
44.00 |
1.40 |
| 8 |
Tang, Adrianna (11)
|
MRLIFE |
45.69 |
44.52 |
-1.17 |
| 9 |
Dunn, Angelina (11)
|
MRENAC |
47.79 |
45.35 |
-2.44 |
| 10 |
Robles, Esme L (12)
|
MRSSC |
46.51 |
45.74 |
-0.77 |
| 11 |
Reedwan, Xiana (11)
|
MRTS |
44.24 |
46.26 |
2.02 |
| 12 |
Buscher, Keely (12)
|
MRHAUP |
48.69 |
46.80 |
-1.89 |
| 13 |
Norris, Kaitlyn O (12)
|
MRHAUP |
49.55 |
47.72 |
-1.83 |
| 14 |
Barberio, Brooke A (11)
|
MRHAUP |
1:10.00 |
47.85 |
-22.15 |
| 15 |
Perez, Isabella B (11)
|
MRCIY |
50.67 |
47.89 |
-2.78 |
| 16 |
Kelly, Ava (11)
|
MRHYB |
57.62 |
48.59 |
-9.03 |
| 17 |
Cortes Morales, Mariana (12)
|
MRHYB |
47.11 |
48.62 |
1.51 |
| 18 |
Deleski, Kate (12)
|
MREHY |
50.11 |
50.13 |
0.02 |
| 19 |
Zabniak, Alexandra * (11)
|
MRTS |
51.10 |
50.17 |
-0.93 |
| 20 |
Intonato, Aurelia A (12)
|
MREHY |
50.40 |
50.18 |
-0.22 |
| 21 |
Viggiano, Ashley R (11)
|
MRSSC |
54.42 |
53.03 |
-1.39 |
| 22 |
Figueroa, Aaliyah R (11)
|
MRSSC |
|
55.23 |
- |
| 23 |
Hagan, Audrey E (11)
|
MRSSC |
58.81 |
57.72 |
-1.09 |
| 24 |
Lewis, Asia M (11)
|
MRSSC |
|
1:03.64 |
- |
| 25 |
Sandberg, Olivia F (11)
|
MRSSC |
|
1:06.47 |
- |
| 25 |
Chinchilla Claros, Mia (11)
|
MRTS |
1:07.95 |
1:06.47 |
-1.48 |
| 27 |
Henry, Janae J (11)
|
MRSSC |
|
1:06.60 |
- |
| 28 |
Thykkoottathil, Isha Bijosh (11)
|
MRSSC |
|
1:10.15 |
- |
| 29 |
Mitchell, Leah N (11)
|
MRHAUP |
1:08.83 |
1:17.69 |
8.86 |
| 30 |
Velupula, Hamsini S (11)
|
MRSSC |
|
1:21.16 |
- |
|
Schnauder, Gianna S (12)
|
MRSSC |
52.01 |
DQ |
- |
|
Nowakowski, Leah J (11)
|
MRSSC |
|
DQ |
- |