| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gervase, Isabella (12)
|
MRHYB |
35.09 |
34.62 |
-0.47 |
| 2 |
Dorgan, Addelyn (12)
|
MRCSDC |
38.02 |
36.15 |
-1.87 |
| 3 |
Reed, Lauren (12)
|
MRHYB |
42.28 |
37.36 |
-4.92 |
| 4 |
Robles, Esme L (12)
|
MRSSC |
40.97 |
37.65 |
-3.32 |
| 5 |
Flaherty, Reagan E (11)
|
MRCSDC |
42.84 |
39.48 |
-3.36 |
| 6 |
Schnauder, Gianna S (12)
|
MRSSC |
50.23 |
41.78 |
-8.45 |
| 7 |
Dwyer, Kenna (11)
|
MRHYB |
43.87 |
43.06 |
-0.81 |
| 8 |
Nowakowski, Leah J (11)
|
MRSSC |
|
43.58 |
- |
| 9 |
Bobkin, Emma R (11)
|
MRTS |
44.58 |
43.94 |
-0.64 |
| 10 |
Figueroa, Aaliyah R (11)
|
MRSSC |
50.46 |
44.69 |
-5.77 |
| 11 |
Norris, Kaitlyn O (12)
|
MRHAUP |
1:00.00 |
45.76 |
-14.24 |
| 12 |
Balcells, Laia G (11)
|
MRSSC |
48.43 |
46.33 |
-2.10 |
| 13 |
McDermott, Charlotte (11)
|
MRHYB |
52.00 |
49.95 |
-2.05 |
| 14 |
Thykkoottathil, Isha Bijosh (11)
|
MRSSC |
|
1:05.24 |
- |
|
Ho, Victoria I (12)
|
MRHAUP |
59.77 |
DQ |
- |