| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sonn, Camille A (12)
|
PVAAC |
42.25 |
42.80 |
0.55 |
| 1 |
Kiang, Vivian Y (12)
|
PVNCAP |
59.01 |
50.47 |
-8.54 |
| 2 |
Harms, Stella J (12)
|
PVNCAP |
41.58 |
42.85 |
1.27 |
| 2 |
Koroma, Camille Y (11)
|
PVNCAP |
49.14 |
50.64 |
1.50 |
| 3 |
Aiken, Eleanor R (11)
|
PVAAC |
45.30 |
43.00 |
-2.30 |
| 3 |
Atala, Emma J (11)
|
PVAAC |
49.50 |
50.82 |
1.32 |
| 4 |
Jones, Sophia P (12)
|
PVNCAP |
45.77 |
43.86 |
-1.91 |
| 4 |
Ledford, Annika S (12)
|
PVAAC |
48.90 |
50.88 |
1.98 |
| 5 |
Pineiro, Carolina S (12)
|
PVAAC |
45.46 |
44.46 |
-1.00 |
| 5 |
Reeves, Sachi M (11)
|
PVPAC |
50.77 |
50.98 |
0.21 |
| 6 |
Shive, Keira X (11)
|
PVNCAP |
46.25 |
48.59 |
2.34 |
| 6 |
Naum, Alexandra S (12)
|
PVNCAP |
51.29 |
51.08 |
-0.21 |
| 7 |
Beidler, Ember L (11)
|
PVAAC |
1:05.97 |
51.73 |
-14.24 |
| 8 |
MacDonald, Mia (11)
|
PVWSH |
55.97 |
51.84 |
-4.13 |
| 9 |
Breton, Leila G (12)
|
PVAAC |
52.65 |
53.78 |
1.13 |
| 10 |
Iraheta, Liliana G (12)
|
PVPGPR |
56.34 |
54.57 |
-1.77 |
| 11 |
Hobbs, Samantha S (11)
|
PVAAC |
50.99 |
55.31 |
4.32 |
| 12 |
La Fleur, Ella C (12)
|
MDACSS |
48.83 |
55.37 |
6.54 |
| 13 |
Hill, Charlotte (11)
|
PVYSS |
59.31 |
56.88 |
-2.43 |
| 14 |
Philson, Riley M (12)
|
PVPGPR |
59.36 |
58.06 |
-1.30 |
| 15 |
McIntyre, Kay H (11)
|
PVAAC |
1:03.12 |
1:00.07 |
-3.05 |
| 16 |
Scarborough, Abigail F (11)
|
PVPAC |
1:04.28 |
1:00.59 |
-3.69 |
| 17 |
Small, Reagan (11)
|
PVYSS |
55.76 |
1:00.62 |
4.86 |
| 18 |
Lakanse, Fiyinfoluwa M (11)
|
PVPGPR |
1:01.02 |
1:00.69 |
-0.33 |
| 19 |
Straub, Charlotte (11)
|
PVYSS |
1:02.00 |
1:03.48 |
1.48 |
| 20 |
von Glahn, Jacqueline (11)
|
PVYSS |
1:03.09 |
1:08.18 |
5.09 |
|
Zhang, Claire X (12)
|
PVWSH |
48.02 |
DQ |
- |
|
Weliwitigoda, Mila A (11)
|
PVNCAP |
49.70 |
NS |
- |
|
Felluca, Evelyn E (12)
|
PVAAC |
|
NS |
- |
|
McKenzie, Ella C (11)
|
PVPGPR |
56.73 |
NS |
- |