| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Young, Lindsay L (15)
|
PVNCAP |
31.44 |
32.35 |
0.91 |
| 2 |
Levine, Lauren F (16)
|
PVNCAP |
32.80 |
33.31 |
0.51 |
| 3 |
Nelson, Katarzyna A (18)
|
PVTANK |
33.02 |
34.61 |
1.59 |
| 4 |
Calandra, Lily A (15)
|
MRSSC |
32.97 |
34.71 |
1.74 |
| 5 |
Watts, Emma C (15)
|
PVFISH |
37.85 |
35.33 |
-2.52 |
| 6 |
Vertichio, Kaylie A (18)
|
MRSSC |
35.86 |
36.70 |
0.84 |
| 7 |
Wood, Olivia S (15)
|
PVNCAP |
36.82 |
37.16 |
0.34 |
| 8 |
McMurry, Natalie C (17)
|
PVTOLL |
37.34 |
38.99 |
1.65 |
| 9 |
Mackay, Alexandria E (17)
|
MRSSC |
37.10 |
39.41 |
2.31 |
| 10 |
Kotek, Annabel R (15)
|
PVTOLL |
39.78 |
41.24 |
1.46 |
| 11 |
Glennon, Abigail K (17)
|
MRSSC |
42.25 |
43.27 |
1.02 |
| 12 |
Mahady, Madison G (15)
|
MRSSC |
44.19 |
43.92 |
-0.27 |
| 13 |
McPhail, Madison R (15)
|
MDHFY |
46.20 |
46.13 |
-0.07 |
|
Schmitt, Katherine E (16)
|
PVNCAP |
40.26 |
NS |
- |
|
Carvallo, Valeria V (18)
|
PVTANK |
38.85 |
NS |
- |
|
Windle, Blythe M (16)
|
PVTOLL |
48.50 |
NS |
- |