| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blayney, Kyla R (8)
|
PVMACH |
44.29 |
41.58 |
-2.71 |
| 2 |
Li, Gloria A (8)
|
PVMACH |
50.76 |
42.49 |
-8.27 |
| 3 |
Zhao, Ellie (8)
|
PVFISH |
54.02 |
45.19 |
-8.83 |
| 4 |
Oei, Adalyn Y (8)
|
PVNCAP |
|
48.08 |
- |
| 5 |
Mello, Molly J (8)
|
PVBWST |
55.90 |
51.33 |
-4.57 |
| 6 |
Russell, Fiona C (8)
|
PVASA |
|
52.97 |
- |
| 7 |
Maxey, Lena V (8)
|
PVNCAP |
58.05 |
53.22 |
-4.83 |
| 8 |
Kibsey, Veronika L (8)
|
PVMACH |
1:01.88 |
53.31 |
-8.57 |
| 9 |
Chu, Chloe Y (8)
|
PVASA |
56.33 |
53.35 |
-2.98 |
| 10 |
Miller, Reagan C (8)
|
PVMACH |
1:06.06 |
54.53 |
-11.53 |
| 11 |
Bloss, Madelyn G (8)
|
PVAAC |
|
54.79 |
- |
| 12 |
Atkinson, Madelyn G (8)
|
PVHACC |
|
1:04.37 |
- |
| 13 |
Shrestha, Pralisha (8)
|
PVMACH |
|
1:05.03 |
- |
| 14 |
Amodio, Gia J (8)
|
PVMACH |
|
1:12.15 |
- |
|
Spaisman, Sloan A (8)
|
PVASA |
|
DQ |
- |
|
Haddad, Joely A (8)
|
PVASA |
54.77 |
DQ |
- |
|
Celentano, Adelaide F (8)
|
PVAAC |
|
NS |
- |