| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Woody, Anna L (13)
|
PVMACH |
2:41.44 |
2:39.32 |
-2.12 |
| 2 |
Chen, Sara L (15)
|
PVMACH |
2:39.38 |
2:40.32 |
0.94 |
| 3 |
Li, Samantha W (14)
|
PVMACH |
2:45.76 |
2:45.61 |
-0.15 |
| 4 |
Chow, Chloe S (13)
|
PVMACH |
2:55.00 |
2:53.25 |
-1.75 |
| 5 |
Arllen, Sydney M (14)
|
PVMACH |
2:55.49 |
2:57.07 |
1.58 |
| 6 |
Gatlin, Emerson E (14)
|
PVMACH |
2:53.56 |
2:58.14 |
4.58 |
| 7 |
Lee-Truesdell, Isabella S (15)
|
PVMACH |
2:56.15 |
3:02.56 |
6.41 |
| 8 |
Paxton, Harper R (13)
|
PVMACH |
|
3:05.26 |
- |
| 9 |
Lorre Moalic, Louxane (13)
|
PVSDS |
3:25.44 |
3:23.52 |
-1.92 |
| 10 |
Cunningham, Whitney A (13)
|
PVMACH |
3:35.16 |
3:38.21 |
3.05 |