| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
White, Lochlyn E (7)
|
PVNCAP |
24.89 |
24.26 |
-0.63 |
| 2 |
Nguyen, Hadley B (6)
|
PVMACH |
27.17 |
25.42 |
-1.75 |
| 3 |
Martin, Rebecca M (7)
|
PVNCAP |
24.79 |
25.62 |
0.83 |
| 4 |
Blayney, Kyla R (7)
|
PVMACH |
27.36 |
26.35 |
-1.01 |
| 5 |
Sumrow, Eliza R (7)
|
PVNCAP |
29.44 |
26.72 |
-2.72 |
| 6 |
Blank, Brinkley C (7)
|
PVNCAP |
26.30 |
27.01 |
0.71 |
| 7 |
McGinley, Madelyn G (7)
|
PVNCAP |
27.45 |
27.75 |
0.30 |
| 8 |
North, Kylie J (7)
|
PVNCAP |
28.57 |
27.78 |
-0.79 |
| 9 |
Vizcaino, Lennon B (7)
|
PVMACH |
|
27.85 |
- |
| 10 |
Simpson, Harper G (6)
|
PVNCAP |
31.20 |
30.81 |
-0.39 |
| 11 |
Hershey, Reagan E (7)
|
PVMACH |
|
30.83 |
- |
| 12 |
Buonforte, Lilia R (7)
|
PVMACH |
31.42 |
31.69 |
0.27 |
| 13 |
Pleisch, Aliyah J (7)
|
PVNCAP |
31.74 |
35.39 |
3.65 |
| 14 |
Cavanaugh, Riley M (7)
|
PVNCAP |
31.27 |
35.99 |
4.72 |
| 15 |
Jaczko, Penrose M (7)
|
PVNCAP |
36.74 |
37.66 |
0.92 |
| 16 |
Roth, Ava G (7)
|
PVMACH |
34.32 |
39.38 |
5.06 |
|
Mays, Hazel M (6)
|
PVNCAP |
|
DQ |
- |
|
Duan, Sarah S (6)
|
PVNCAP |
|
DQ |
- |
|
Radford, Gabrielle L (7)
|
PVNCAP |
30.94 |
DQ |
- |
|
Roper, Amelia G (7)
|
PVNCAP |
39.33 |
DQ |
- |
|
Sverchek, Josephine C (7)
|
PVMACH |
|
DQ |
- |
|
Gadwale, Anjali G (7)
|
PVNCAP |
|
NS |
- |