| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tempelis, Marie E (7)
|
PVFXFX |
45.86 |
43.54 |
-2.32 |
| 2 |
Zhao, Ellie (7)
|
PVFISH |
43.91 |
43.60 |
-0.31 |
| 3 |
Sanglier, Chloe A (7)
|
PVPM |
48.15 |
46.46 |
-1.69 |
| 4 |
McAsey, Caroline J (7)
|
PVPM |
|
47.36 |
- |
| 5 |
Fatah, Aria S (7)
|
PVPM |
50.17 |
51.66 |
1.49 |
| 6 |
Zerbe, Mason E (7)
|
PVFISH |
52.82 |
53.12 |
0.30 |
| 7 |
Beckett, Maali M (7)
|
PVPM |
|
53.85 |
- |
| 8 |
Cook, Penelope R (7)
|
PVPM |
53.33 |
54.26 |
0.93 |
| 9 |
MacDonald, Mikayla N (7)
|
PVWSH |
1:06.33 |
55.46 |
-10.87 |
| 10 |
Mahaffy, Elizabeth K (7)
|
PVFISH |
59.85 |
1:00.00 |
0.15 |
| 11 |
Cunneen, Julia M (7)
|
PVPM |
1:09.07 |
1:05.13 |
-3.94 |
| 12 |
Yi, Victoria J (7)
|
PVFISH |
1:33.68 |
1:21.41 |
-12.27 |
|
McCarrison, Kori J (7)
|
PVPM |
1:04.77 |
DQ |
- |
|
Camet, Yzabella Grace S (7)
|
PVPM |
1:06.81 |
NS |
- |
|
Mohtashamian, Darya S (7)
|
PVFISH |
1:08.77 |
NS |
- |
|
Zhang, Claire Z (7)
|
PVFISH |
|
NS |
- |