| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Layne C (16)
|
VACCA |
2:32.07 |
2:39.37 |
7.30 |
| 2 |
McLaughlin, Delaney L (17)
|
VACCA |
2:33.01 |
2:41.01 |
8.00 |
| 3 |
Spurlock, Avery M (15)
|
VACCA |
2:33.43 |
2:41.62 |
8.19 |
| 4 |
Heale, Annie C (14)
|
VASMAC |
2:46.53 |
2:47.18 |
0.65 |
| 5 |
White, Jakiah I (14)
|
VASMAC |
2:48.28 |
2:47.49 |
-0.79 |
| 6 |
Carter, Emmi D (15)
|
VACCA |
2:45.85 |
2:55.55 |
9.70 |
| 7 |
Ayers, Ansley E (17)
|
VACCA |
2:47.89 |
2:55.58 |
7.69 |
| 8 |
Pullen, Alexis L (14)
|
VASMAC |
3:00.38 |
2:58.20 |
-2.18 |
| 9 |
Coleman, Tess H (16)
|
VACCA |
2:48.86 |
2:59.77 |
10.91 |
| 10 |
Sterner, Shayla F (13)
|
VASMAC |
3:09.08 |
3:09.27 |
0.19 |
| 11 |
Nylander, Emma W (13)
|
VASMAC |
3:14.03 |
3:16.43 |
2.40 |
| 12 |
Floyd, Caroline R (14)
|
VACCA |
3:33.45 |
3:21.75 |
-11.70 |
| 13 |
Mandujano, Liliana I (13)
|
VASMAC |
3:23.81 |
3:23.87 |
0.06 |
| 14 |
Nutter, Lillian E (13)
|
VAGATR |
3:37.69 |
3:32.91 |
-4.78 |
| 15 |
White, Jaliyah E (13)
|
VASMAC |
3:33.11 |
3:35.79 |
2.68 |
| 16 |
Hartley, Madeline G (13)
|
VASMAC |
3:39.42 |
3:46.02 |
6.60 |
| 17 |
Howitt, Kennedy B (13)
|
VACCA |
3:45.77 |
3:47.05 |
1.28 |
|
Gilhooly, Teagan M (14)
|
VAHNVR |
2:49.72 |
NS |
- |