| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spurlock, Avery M (15)
|
VACCA |
32.89 |
33.09 |
0.20 |
| 2 |
McLaughlin, Delaney L (17)
|
VACCA |
35.08 |
34.06 |
-1.02 |
| 3 |
Jones, Layne C (16)
|
VACCA |
|
34.22 |
- |
| 4 |
Warren, Surai B (13)
|
VARCR |
39.18 |
35.14 |
-4.04 |
| 5 |
Pullen, Alexis L (14)
|
VASMAC |
46.84 |
36.73 |
-10.11 |
| 6 |
Coleman, Tess H (16)
|
VACCA |
|
37.32 |
- |
| 7 |
Ayers, Ansley E (17)
|
VACCA |
39.08 |
37.40 |
-1.68 |
| 8 |
Zyglocke, Lilly M (13)
|
VAHNVR |
36.01 |
37.49 |
1.48 |
| 9 |
Wayner, Sophia H (15)
|
VACA-Y |
37.97 |
37.67 |
-0.30 |
| 10 |
Mandujano, Liliana I (13)
|
VASMAC |
45.47 |
39.39 |
-6.08 |
| 11 |
Sterner, Shayla F (13)
|
VASMAC |
48.72 |
39.88 |
-8.84 |
| 12 |
McCatty, Camden I (13)
|
VARCR |
50.21 |
53.94 |
3.73 |
|
Gilhooly, Teagan M (14)
|
VAHNVR |
34.88 |
NS |
- |
|
Stevens, Jenna L (15)
|
VAHNVR |
33.22 |
NS |
- |