| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burton, Abigail R (12)
|
NCEAC |
2:32.26 |
2:34.49 |
2.23 |
| 2 |
Dameron, Finley K (12)
|
VA757 |
2:46.62 |
2:51.55 |
4.93 |
| 3 |
Schlereth, Emily T (12)
|
MRCOND |
2:48.18 |
2:52.51 |
4.33 |
| 4 |
Gustafson, Camryn G (12)
|
VASRVA |
|
2:54.86 |
- |
| 5 |
Vary, Emily E (12)
|
NCEAC |
|
2:58.15 |
- |
| 6 |
Trevino, Gabriella B (11)
|
NCEAC |
|
2:59.32 |
- |
| 7 |
Eisenhart, Abigail H (12)
|
PVNCAP |
3:18.26 |
2:59.57 |
-18.69 |
| 8 |
Weber, Eden F (11)
|
VASRVA |
3:07.15 |
3:01.41 |
-5.74 |
| 9 |
Narkawicz, Eleanor L (11)
|
VACGBD |
2:59.62 |
3:01.51 |
1.89 |
| 10 |
Mueller, Taylor N (11)
|
VACGBD |
3:02.14 |
3:08.99 |
6.85 |
| 11 |
Welch, Madeline L (12)
|
VACGBD |
3:26.81 |
3:18.78 |
-8.03 |
| 12 |
Morgan, Caroline H (12)
|
VASRVA |
3:18.48 |
3:27.21 |
8.73 |
| 13 |
Braganza, Elia Lyra A (12)
|
VACGBD |
3:33.15 |
3:28.94 |
-4.21 |
| 14 |
Kadunc, Laura A (11)
|
VASRVA |
3:37.24 |
3:39.10 |
1.86 |
| 15 |
Rawls, Emma N (12)
|
VASRVA |
4:20.09 |
3:51.20 |
-28.89 |
|
Margulies, Rachel A (11)
|
MRCOND |
|
DQ |
- |