| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bao, Hanyin (10)
|
MRCOND |
2:44.87 |
2:49.84 |
4.97 |
| 2 |
Kerstetter, Callie M (10)
|
VACGBD |
3:12.40 |
2:59.37 |
-13.03 |
| 3 |
James, Hadley M (10)
|
VA757 |
3:03.29 |
3:00.70 |
-2.59 |
| 4 |
Mays, Grace E (10)
|
PVNCAP |
3:13.01 |
3:05.54 |
-7.47 |
| 5 |
Radford, Callie A (9)
|
PVNCAP |
3:11.65 |
3:06.34 |
-5.31 |
| 6 |
Hutto, Addison P (10)
|
VACGBD |
3:21.17 |
3:07.80 |
-13.37 |
| 7 |
Paska, Claire R (10)
|
PVNCAP |
|
3:10.36 |
- |
| 8 |
Austin, Skyler L (10)
|
VA757 |
|
3:10.80 |
- |
| 9 |
Amini, Arria (10)
|
VACGBD |
3:10.69 |
3:15.49 |
4.80 |
| 10 |
McHose, Eleanor (9)
|
VA757 |
3:19.45 |
3:21.94 |
2.49 |
| 11 |
Scianandre, Gina G (10)
|
VA757 |
|
3:25.99 |
- |
| 12 |
Brown, Paige L (10)
|
VASRVA |
3:31.03 |
3:26.98 |
-4.05 |
| 13 |
Shipe, Chloe E (10)
|
VACGBD |
3:46.39 |
3:34.18 |
-12.21 |
| 14 |
Almassian, Laleh R (9)
|
PVNCAP |
3:49.36 |
3:34.99 |
-14.37 |
| 15 |
Strickland, Lydia C (9)
|
VASRVA |
|
3:49.40 |
- |
| 16 |
Hairfield, Alexandra G (9)
|
VASRVA |
|
3:51.75 |
- |