| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spurgeon, Mary E (11)
|
ADSCHE |
46.90 |
44.90 |
-2.00 |
| 2 |
Ryan, Quinlan A (10)
|
ADCPP |
44.44 |
45.60 |
1.16 |
| 3 |
Harder, Phoebe C (11)
|
ADUSAM |
43.65 |
45.71 |
2.06 |
| 4 |
Sanghi, Ingrid K (10)
|
ADCPP |
44.37 |
48.57 |
4.20 |
| 5 |
Craig, Kylie A (11)
|
ADSCHE |
48.32 |
49.44 |
1.12 |
| 6 |
Miller, Braelyn G (11)
|
NESNAP |
51.05 |
49.60 |
-1.45 |
| 7 |
Zima, Adelaide G (10)
|
ADSCHE |
49.16 |
50.71 |
1.55 |
| 8 |
Sayre, Charlotte P (10)
|
ADALBS |
55.19 |
52.40 |
-2.79 |
| 9 |
Nimmagadda, Sloka (11)
|
ADALBS |
54.73 |
54.22 |
-0.51 |
| 10 |
Hu, Ruihan C (10)
|
ADCPP |
56.64 |
55.85 |
-0.79 |
| 11 |
Wang, Phira Y (10)
|
ADCPP |
1:01.65 |
55.88 |
-5.77 |
| 12 |
Casalinuovo, Brynn M (11)
|
ADCPP |
1:03.73 |
59.40 |
-4.33 |
| 13 |
Cahill, Sloane M (10)
|
ADSCHE |
58.37 |
1:01.04 |
2.67 |
| 14 |
Gu, Alyssa (10)
|
ADCPP |
1:07.47 |
1:03.46 |
-4.01 |
| 15 |
li, Bella Q (10)
|
ADCPP |
|
1:03.68 |
- |
|
Silvestre, Maya Bettina M (11)
|
ADCPP |
|
DQ |
- |