| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brecke, Avery M (14)
|
VATIDE |
29.38 |
29.15 |
-0.23 |
| 2 |
Pelletier, Zoey N (14)
|
VACGBD |
29.34 |
29.53 |
0.19 |
| 3 |
Sydorchenko, Kateryna (14)
|
NCTEAM |
30.40 |
29.67 |
-0.73 |
| 4 |
Mannato, Alexis L (14)
|
VATIDE |
30.63 |
29.68 |
-0.95 |
| 5 |
Eberle, Layla G (14)
|
VATIDE |
30.39 |
29.88 |
-0.51 |
| 6 |
Patterson, Madelynn A (14)
|
VATIDE |
29.56 |
29.90 |
0.34 |
| 7 |
Chung, Olivia D (13)
|
VACGBD |
29.37 |
29.94 |
0.57 |
| 8 |
Watson, Viktoria L (14)
|
VACGBD |
31.26 |
30.07 |
-1.19 |
| 9 |
Kawecki, Lillian C (13)
|
VACGBD |
32.30 |
30.21 |
-2.09 |
| 10 |
Eberle, Emery F (13)
|
VATIDE |
|
30.29 |
- |
| 11 |
Landon, Emma M (14)
|
VAECAT |
31.35 |
30.30 |
-1.05 |
| 12 |
LaFlamme, Zoe L (13)
|
VACGBD |
30.20 |
30.54 |
0.34 |
| 13 |
Jones, Kyrsten C (14)
|
VATIDE |
30.91 |
30.72 |
-0.19 |
| 14 |
Heustis, Bailey M (14)
|
VAODAC |
33.06 |
30.77 |
-2.29 |
| 15 |
Dela Cruz, Rianna Almira V (14)
|
VATIDE |
31.08 |
30.97 |
-0.11 |
| 16 |
Gordon, Madelyn L (14)
|
VAUN-O |
30.30 |
31.74 |
1.44 |