| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vanyo, Vivian R (10)
|
VAACAC |
33.99 |
32.93 |
-1.06 |
| 2 |
Kadela, Charlotte S (10)
|
VACCA |
35.57 |
32.95 |
-2.62 |
| 3 |
Sessa, Victoria I (10)
|
VANOVA |
34.92 |
33.13 |
-1.79 |
| 4 |
Nolde, Anna E (10)
|
VACA-Y |
34.24 |
33.85 |
-0.39 |
| 5 |
Codington, Emmylou L (10)
|
VATIDE |
36.62 |
34.42 |
-2.20 |
| 5 |
Browning, Olivia C (10)
|
VANOVA |
36.33 |
34.42 |
-1.91 |
| 7 |
Kadela, Isabelle M (10)
|
VACCA |
36.61 |
34.47 |
-2.14 |
| 8 |
Li, Chelsea T (10)
|
VATIDE |
35.30 |
34.55 |
-0.75 |
| 9 |
Davila, Naomi J (10)
|
VAPWSC |
35.57 |
34.60 |
-0.97 |
| 10 |
Crone, Hayes T (10)
|
VANOVA |
34.99 |
35.29 |
0.30 |
| 11 |
Sabo, Molly A (10)
|
VAHNVR |
35.03 |
35.35 |
0.32 |
| 12 |
Kawecki, Daisy C (10)
|
VACGBD |
36.34 |
35.43 |
-0.91 |
| 13 |
Betsworth, Emory J (10)
|
VACA-Y |
36.35 |
35.52 |
-0.83 |
| 14 |
Ferguson, Abigail E (9)
|
VARAYS |
35.78 |
35.58 |
-0.20 |
| 15 |
Minson, Alivia R (10)
|
VACGBD |
36.27 |
35.90 |
-0.37 |
| 16 |
Tsybulina, Yara E (9)
|
VANOVA |
35.73 |
36.02 |
0.29 |
| 17 |
Rawles, Katherine M (10)
|
VADC |
36.10 |
36.03 |
-0.07 |
| 18 |
Goins, Elizabeth K (8)
|
VANOVA |
36.62 |
36.38 |
-0.24 |
| 19 |
Edmunds, Harper D (10)
|
VANOVA |
35.74 |
36.48 |
0.74 |
| 20 |
Avis, Anne Tillison T (10)
|
VACCA |
36.33 |
36.71 |
0.38 |
| 21 |
Luna, Lillian E (9)
|
VAODAC |
36.69 |
36.72 |
0.03 |
| 22 |
Hitt, Virginia-Annabelle H (10)
|
VACA-Y |
35.90 |
36.75 |
0.85 |
| 23 |
McQueen, Amaya K (10)
|
VAQSTS |
35.27 |
37.40 |
2.13 |
| 24 |
Grossman, Sloane A (10)
|
VANOVA |
35.58 |
37.45 |
1.87 |
| 25 |
Johnson, Chloe M (10)
|
VATIDE |
35.47 |
37.65 |
2.18 |
| 26 |
Royster, Brielle V (10)
|
VANOVA |
36.49 |
37.68 |
1.19 |
| 27 |
Maust, Claire E (10)
|
VAGATR |
36.67 |
38.12 |
1.45 |
| 28 |
Martin, Margaret M (10)
|
VAHOKI |
36.33 |
38.17 |
1.84 |
| 29 |
Boyd, Caroline T (10)
|
VANOVA |
36.42 |
38.33 |
1.91 |