| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blanco, Sophia L (12)
|
PVFAST |
32.56 |
32.48 |
-0.08 |
| 2 |
McGill, Rylan (12)
|
PVPGPR |
33.87 |
32.77 |
-1.10 |
| 3 |
Manuel, Samantha G (12)
|
PVASTS |
33.77 |
34.25 |
0.48 |
| 4 |
Hugee, Raya (11)
|
PVPGPR |
37.09 |
36.18 |
-0.91 |
| 5 |
Senior, Hannah S (12)
|
PVASTS |
39.70 |
39.41 |
-0.29 |
| 6 |
Senior, Michaela K (11)
|
PVASTS |
44.09 |
40.18 |
-3.91 |
| 7 |
Aitelhabib, Habiba (11)
|
PVASTS |
42.35 |
40.73 |
-1.62 |
| 8 |
Lakanse, Fiyinfoluwa M (12)
|
PVPGPR |
41.00 |
40.87 |
-0.13 |
| 9 |
Reaves, Raea (12)
|
PVPGPR |
43.63 |
41.13 |
-2.50 |
| 10 |
Wheeler, Kennedi V (11)
|
PVASTS |
41.55 |
42.07 |
0.52 |
| 11 |
Rogers, Toure (11)
|
PVASTS |
53.76 |
47.57 |
-6.19 |
|
Black, Kaylee D (11)
|
PVASTS |
|
NS |
- |
|
Loftus, Grace K (11)
|
PVASTS |
32.06 |
NS |
- |
|
Josiger, Emily A (11)
|
PVFAST |
50.30 |
NS |
- |
|
Pierola, Alessandra A (11)
|
PVFAST |
36.08 |
NS |
- |