| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yu, Christopher B (14)
|
MDEST |
40.66 |
34.54 |
-6.12 |
| 2 |
Linehan, Foster L (14)
|
MDSPY |
38.12 |
35.09 |
-3.03 |
| 3 |
Spencer, Aidan R (13)
|
MDEST |
44.90 |
39.39 |
-5.51 |
| 4 |
Liu, Ethan (14)
|
MDRAC |
44.10 |
41.45 |
-2.65 |
| 5 |
Marwaha, Rajan B (14)
|
MDEST |
44.88 |
41.74 |
-3.14 |
| 6 |
Alliger, Evan P (14)
|
MDRAC |
47.97 |
42.21 |
-5.76 |
| 7 |
Shepelevych, Nazar (13)
|
MDRAC |
45.89 |
43.84 |
-2.05 |
| 8 |
Barnett, Jason R (14)
|
MDBCSC |
38.57 |
44.75 |
6.18 |
| 9 |
Bettegowda, Sachin (13)
|
MDMAS |
47.45 |
45.83 |
-1.62 |
| 10 |
Lin, Zicheng (13)
|
MDMAS |
42.03 |
47.34 |
5.31 |
| 11 |
Conley, Richard P (14)
|
MDMAS |
59.65 |
48.29 |
-11.36 |
| 12 |
Hart, Anderson (13)
|
MDGTAC |
|
49.25 |
- |
| 13 |
Larbalestrier-Conawa, Benja (13)
|
MDSPY |
52.24 |
51.07 |
-1.17 |
| 14 |
Shorter, Nathan C (13)
|
MDNBAC |
1:02.51 |
53.03 |
-9.48 |
| 15 |
Garrett, Seeley M (13)
|
MDESA |
53.52 |
55.82 |
2.30 |
| 16 |
Meteer, Charles T (13)
|
MDGTAC |
53.21 |
1:01.28 |
8.07 |
|
Davis, Christopher J (13)
|
MDSPY |
48.37 |
DQ |
- |
|
Ervick, Austin R (13)
|
MDSPY |
47.24 |
DQ |
- |