| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thomas, Emma M (13)
|
PVFXFX |
26.16 |
27.40 |
1.24 |
| 2 |
Walton, Lilian J (13)
|
PVDCPR |
28.52 |
28.38 |
-0.14 |
| 3 |
Neppl, Ridgely E (13)
|
PVFXFX |
28.79 |
28.87 |
0.08 |
| 4 |
Beggiato, Eva M (14)
|
PVDCPR |
28.51 |
29.04 |
0.53 |
| 5 |
Thamasett, Elizabeth M (14)
|
PVFXFX |
30.05 |
29.66 |
-0.39 |
| 6 |
Alliger, Avery (14)
|
MDACSS |
30.38 |
29.81 |
-0.57 |
| 7 |
Hickman, Addison (13)
|
PVTOLL |
|
30.04 |
- |
| 8 |
Charles-Balthazar, Danariell (14)
|
PVDCPR |
|
30.54 |
- |
| 9 |
Podlesny, Nina (13)
|
PVJFD |
31.21 |
30.73 |
-0.48 |
| 10 |
Norris, Brooke (13)
|
WVSUWC |
30.85 |
30.75 |
-0.10 |
| 11 |
Cowperthwaite, Abigail M (14)
|
PVPAC |
31.42 |
31.52 |
0.10 |
| 12 |
Guhr, Kajsa J (14)
|
PVDCPR |
32.31 |
31.66 |
-0.65 |
| 13 |
Dillon, Charlotte T (13)
|
PVDCPR |
33.01 |
31.70 |
-1.31 |
| 14 |
Soloway, Lucie J (13)
|
PVDCPR |
34.01 |
32.45 |
-1.56 |
| 15 |
Kim, Abigail Y (14)
|
PVTRA |
32.92 |
33.09 |
0.17 |
| 16 |
Minalu, Kalkidan A (13)
|
PVDCPR |
33.82 |
33.25 |
-0.57 |
| 17 |
Raspberry-Hallmon, Riley S (14)
|
PVDCPR |
33.73 |
33.98 |
0.25 |
| 18 |
Titherington, Harper J (13)
|
PVDCPR |
34.88 |
34.30 |
-0.58 |
| 19 |
Arcara, Eloise S (14)
|
PVDCPR |
33.40 |
34.81 |
1.41 |
| 20 |
Myles, Amirah J (13)
|
PVDCPR |
|
35.60 |
- |
| 21 |
Ellsworth, Sloan K (14)
|
PVDCPR |
35.96 |
35.77 |
-0.19 |
| 22 |
Bunn, Logan M (13)
|
PVDCPR |
36.28 |
36.02 |
-0.26 |
| 23 |
Maiberger, Madeline D (13)
|
PVJFD |
36.23 |
36.76 |
0.53 |
| 24 |
Roston, Alexandra R (13)
|
PVDCPR |
|
37.41 |
- |
| 25 |
Blanton, Mary Rose L (13)
|
PVDCPR |
37.99 |
38.20 |
0.21 |
| 26 |
Grim, Iris G (13)
|
PVDCPR |
37.50 |
38.83 |
1.33 |
| 27 |
Coleman, Charley D (13)
|
PVDCPR |
|
39.67 |
- |
| 28 |
Deal, Aubrey D (13)
|
WVSUWC |
40.30 |
39.82 |
-0.48 |
|
Shah, Ria P (13)
|
PVDCPR |
35.92 |
NS |
- |
|
Raney Ferrero, Uli S (14)
|
PVDCPR |
|
NS |
- |
|
Beckmann, Elisabeth G (13)
|
PVPAC |
36.74 |
NS |
- |