| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fenton, Frances E (16)
|
VALY |
54.32 |
55.24 |
0.92 |
| 2 |
Ismailova, Diana (15)
|
VACA-Y |
53.14 |
55.76 |
2.62 |
| 3 |
Ward, Ruthie E (17)
|
VAVABR |
55.41 |
58.73 |
3.32 |
| 4 |
Mills, Anna M (16)
|
VALY |
56.98 |
58.76 |
1.78 |
| 5 |
Hoang, Angelina J (16)
|
VACA-Y |
56.20 |
59.13 |
2.93 |
| 6 |
Kim, Hannah (15)
|
VALY |
1:00.38 |
59.18 |
-1.20 |
| 7 |
Ward, Alena F (16)
|
VAVABR |
54.78 |
59.35 |
4.57 |
| 8 |
Fleenor, Elizabeth G (17)
|
VAVABR |
56.09 |
1:01.62 |
5.53 |
| 9 |
Nicely, Olivia G (16)
|
VAVABR |
1:01.35 |
1:01.97 |
0.62 |
| 10 |
Conforti-Johnson, Sophia K (16)
|
VAVABR |
58.67 |
1:04.30 |
5.63 |
| 11 |
Judy, Emily A (16)
|
VALY |
54.58 |
1:04.80 |
10.22 |
| 12 |
Kirby, Ava G (16)
|
VALY |
1:06.59 |
1:07.74 |
1.15 |
| 13 |
Asbury, Emily A (16)
|
VALY |
1:13.05 |
1:15.93 |
2.88 |