| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eanes, Kellyn Z (10)
|
VAFCFY |
32.36 |
32.98 |
0.62 |
| 2 |
Smith, Jaylen M (10)
|
VAVABR |
33.08 |
33.19 |
0.11 |
| 3 |
Shaw, Sunrise (10)
|
VACA-Y |
33.55 |
33.24 |
-0.31 |
| 4 |
Greenlaw, Camille A (10)
|
VALY |
33.39 |
33.55 |
0.16 |
| 5 |
Keith, Lana J (10)
|
VALY |
36.63 |
35.70 |
-0.93 |
| 6 |
Eakkhattiyakorn, Tamara (10)
|
VALY |
37.24 |
36.63 |
-0.61 |
| 7 |
Morrison, Dabney E (9)
|
VALY |
37.64 |
37.09 |
-0.55 |
| 8 |
Honaker, Shae E (9)
|
VAVABR |
38.37 |
37.32 |
-1.05 |
| 9 |
Harper, Eliza P (10)
|
VAVABR |
38.14 |
37.52 |
-0.62 |
| 10 |
Schnetzler, Patricia B (9)
|
VAVABR |
38.08 |
37.57 |
-0.51 |
| 12 |
Pickeral, Charlotte R (9)
|
VALY |
37.36 |
38.00 |
0.64 |
| 13 |
Britt, Sarah Kate (10)
|
VACA-Y |
37.63 |
38.32 |
0.69 |
| 14 |
Bradshaw, Piper R (9)
|
VACA-Y |
37.68 |
38.88 |
1.20 |
| 15 |
Robbins, Elizabeth R (10)
|
VACA-Y |
40.79 |
39.44 |
-1.35 |
| 16 |
Torrence, Brianna R (10)
|
VALY |
38.17 |
40.35 |
2.18 |
| 24 |
Foley, Laine M (9)
|
VAVABR |
49.84 |
49.80 |
-0.04 |
| 27 |
Thomas, Alaina R (9)
|
VALY |
51.37 |
58.29 |
6.92 |
|
Buck, Braelyn V (9)
|
VAVABR |
46.72 |
NS |
- |
|
Owen, Campbell L (10)
|
VALY |
50.61 |
NS |
- |