| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Noah E (14)
|
MDFAST |
28.52 |
28.50 |
-0.02 |
| 2 |
Marsh-Brandes, Daniel R (13)
|
MDFAST |
30.85 |
30.48 |
-0.37 |
| 3 |
Wolfson, Rhys J (14)
|
MDMBK |
32.21 |
30.93 |
-1.28 |
| 4 |
Asare, Joel C (14)
|
MDFAST |
35.02 |
31.22 |
-3.80 |
| 5 |
Kraeuter, Paul F (13)
|
MDOPST |
33.66 |
33.05 |
-0.61 |
| 6 |
De Los Reyes-Kundey, Aden A (13)
|
MDMAC |
36.27 |
33.21 |
-3.06 |
| 7 |
Segovia, Jayden A (14)
|
MDFAST |
34.27 |
33.99 |
-0.28 |
| 7 |
Wirth, Micah C (13)
|
MDFAST |
33.93 |
33.99 |
0.06 |
| 9 |
Begazo, Gianpierre R (13)
|
MDMAC |
38.33 |
34.32 |
-4.01 |
| 10 |
Kim, Colin S (14)
|
MDYCM |
37.92 |
34.85 |
-3.07 |
| 11 |
Thompson, Gavin Q (13)
|
MDMAC |
41.41 |
35.46 |
-5.95 |
| 12 |
Greene, Gavin (13)
|
MDBAY |
36.74 |
35.61 |
-1.13 |
| 13 |
Tkachenko, Eric (13)
|
MDCAA |
36.75 |
36.91 |
0.16 |
| 14 |
Cocker, Bryce A (14)
|
MDMBK |
39.27 |
36.97 |
-2.30 |
| 15 |
Suprun, Maximilian E (13)
|
MDCAA |
35.32 |
37.96 |
2.64 |
| 16 |
Thompson, Joshua-David (13)
|
MDBAY |
43.89 |
40.97 |
-2.92 |
| 17 |
Witt, Logan (14)
|
MDYCM |
48.29 |
45.42 |
-2.87 |
|
Marsh-Brandes, Alexander E (14)
|
MDFAST |
30.11 |
NS |
- |
|
Coulbourne, Caleb K (13)
|
MDCGA |
37.17 |
NS |
- |
|
Karnati, Aryaneil R (13)
|
MDYCM |
35.66 |
NS |
- |
|
Wilson, Ellis (13)
|
MDBAY |
|
NS |
- |
|
Nkongolo, Solomon J (13)
|
MDYCM |
32.26 |
NS |
- |