| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Henriquez, Alexa (10)
|
MRFLY |
37.17 |
36.98 |
-0.19 |
| 2 |
Sieber, Ariel M (10)
|
MRCIY |
38.73 |
37.60 |
-1.13 |
| 3 |
Fagot, Daniellyn (10)
|
MRFLY |
38.22 |
37.84 |
-0.38 |
| 4 |
Ulloa, Ivania A (10)
|
MRFLY |
39.87 |
41.71 |
1.84 |
| 5 |
Ospina, Alicia (10)
|
MRFLY |
43.75 |
42.74 |
-1.01 |
| 6 |
Dellagiovanna, Eva E (10)
|
MRCIY |
47.27 |
43.35 |
-3.92 |
| 7 |
Ruiter, Sterling (10)
|
MRRED |
46.59 |
44.16 |
-2.43 |
| 8 |
Vallejo, Penelope (9)
|
MRFLY |
47.40 |
44.36 |
-3.04 |
| 9 |
Wang, Jayka (9)
|
MRFLY |
47.27 |
45.15 |
-2.12 |
| 10 |
Herrera, Sasha G (10)
|
MRCIY |
47.38 |
45.87 |
-1.51 |
| 11 |
Choi, Bella K (9)
|
MRCIY |
51.53 |
47.63 |
-3.90 |
| 12 |
Fenton, Fiona D (10)
|
MRCIY |
53.21 |
48.24 |
-4.97 |
| 13 |
Soria, Evita (8)
|
MRFLY |
57.33 |
48.87 |
-8.46 |
| 14 |
Verdezoto Y., Sammy J (10)
|
MRRED |
50.56 |
50.40 |
-0.16 |
| 15 |
Wiygul, Clementina L (9)
|
MRFLY |
|
50.54 |
- |
| 16 |
Delinois, Ruby (9)
|
MRRED |
|
51.09 |
- |
| 17 |
Cumba, Kerenh E (9)
|
MRRED |
52.71 |
53.02 |
0.31 |
| 18 |
Colbert, Sage (7)
|
MRRED |
1:00.00 |
57.80 |
-2.20 |
| 19 |
Collins-Shurland, Isabella S (10)
|
MRCIY |
1:02.60 |
1:00.85 |
-1.75 |
|
Zhong, Gloria (6)
|
MRFLY |
|
DQ |
- |