| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wong, Madeleine C (13)
|
MRFLY |
27.94 |
28.63 |
0.69 |
| 2 |
Campese, Jacqueline T (14)
|
MRCIY |
28.60 |
28.80 |
0.20 |
| 3 |
Leal, Jazmine (13)
|
MRRED |
28.72 |
29.09 |
0.37 |
| 4 |
Trinidad, Chelanny A (14)
|
MRRED |
29.65 |
29.39 |
-0.26 |
| 5 |
Guan, Chelsea (13)
|
MRFLY |
28.60 |
29.76 |
1.16 |
| 6 |
Huang, Isabell (14)
|
MRFLY |
28.94 |
30.10 |
1.16 |
| 7 |
Minchala, Emily D (13)
|
MRFLY |
29.86 |
30.12 |
0.26 |
| 8 |
Graham, Calliope (13)
|
MRRED |
30.00 |
30.13 |
0.13 |
| 9 |
McNally, Jessica J (14)
|
MRCIY |
30.83 |
30.14 |
-0.69 |
| 10 |
Menard, Sophia E (13)
|
MRCIY |
30.49 |
30.24 |
-0.25 |
| 11 |
Menard, Madelyn X (14)
|
MRCIY |
29.99 |
30.66 |
0.67 |
| 12 |
Han, Marianne H (14)
|
MRFLY |
30.21 |
30.82 |
0.61 |
| 13 |
Lama, Kesang J (13)
|
MRFLY |
31.30 |
31.18 |
-0.12 |
| 14 |
Tsampas, Zoe A (13)
|
MRCIY |
29.90 |
31.74 |
1.84 |
| 15 |
Lim, Kaylie (14)
|
MRFLY |
32.39 |
31.95 |
-0.44 |
| 16 |
Jaquez, Camila (13)
|
MRRED |
32.49 |
32.55 |
0.06 |
| 17 |
Prevatt- Dillah, Amelia (13)
|
MRRED |
33.81 |
33.36 |
-0.45 |
| 18 |
Wiygul, Julieta V (13)
|
MRFLY |
34.65 |
33.83 |
-0.82 |
| 19 |
Garcia, Scarlett S (14)
|
MRFLY |
34.91 |
34.54 |
-0.37 |
| 20 |
Shneerson, Ilona (13)
|
MRRED |
33.60 |
34.94 |
1.34 |
|
Tempesta, Lucy E (13)
|
MRFLY |
32.98 |
NS |
- |