| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trinidad, Mianny (17)
|
MRRED |
29.19 |
29.32 |
0.13 |
| 2 |
Wright, Ellen J (16)
|
MRRED |
30.16 |
30.02 |
-0.14 |
| 3 |
Soulantzos, Josephine M (15)
|
MRFLY |
|
31.93 |
- |
| 4 |
Lin, Wenxi (16)
|
MRCIY |
33.02 |
33.32 |
0.30 |
| 5 |
Minchala, Lesly Y (18)
|
MRFLY |
33.00 |
33.41 |
0.41 |
| 6 |
McKinney, Emilia W (15)
|
MRCIY |
33.74 |
33.72 |
-0.02 |
| 7 |
Kohm, Sienna C (15)
|
MRCIY |
33.37 |
34.88 |
1.51 |
| 8 |
Sandoval, Joselyn (15)
|
MRRED |
33.94 |
35.23 |
1.29 |
| 9 |
Ng, Tiffany (17)
|
MRCIY |
32.21 |
36.16 |
3.95 |
| 10 |
Heerey, Karolina A (15)
|
MRCIY |
35.67 |
36.35 |
0.68 |
| 11 |
Rojas, Denisse (16)
|
MRFLY |
48.63 |
41.81 |
-6.82 |
| 12 |
Arcentales, Daffnia J (16)
|
MRFLY |
44.89 |
42.73 |
-2.16 |
| 13 |
Zhong, Runjia (15)
|
MRFLY |
|
47.47 |
- |
| 14 |
Tavares, Samantha A (15)
|
MRTSC |
|
50.76 |
- |