| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sieber, Ariel M (10)
|
MRCIY |
42.04 |
38.45 |
-3.59 |
| 2 |
Henriquez, Alexa (10)
|
MRFLY |
38.03 |
39.21 |
1.18 |
| 3 |
Fagot, Daniellyn (10)
|
MRFLY |
39.74 |
39.83 |
0.09 |
| 4 |
Vallejo, Penelope (9)
|
MRFLY |
42.50 |
42.76 |
0.26 |
| 5 |
Ulloa, Ivania A (10)
|
MRFLY |
40.64 |
43.32 |
2.68 |
| 6 |
Dellagiovanna, Eva E (10)
|
MRCIY |
45.72 |
45.31 |
-0.41 |
| 7 |
Ospina, Alicia (10)
|
MRFLY |
48.31 |
47.61 |
-0.70 |
| 8 |
Verdezoto Y., Sammy J (10)
|
MRRED |
|
53.57 |
- |
| 9 |
Herrera, Sasha G (10)
|
MRCIY |
|
53.68 |
- |
| 10 |
Ruiter, Sterling (10)
|
MRRED |
51.67 |
55.44 |
3.77 |
| 11 |
Soria, Evita (8)
|
MRFLY |
52.46 |
55.46 |
3.00 |
| 12 |
Fenton, Fiona D (10)
|
MRCIY |
59.19 |
58.58 |
-0.61 |
| 13 |
Wang, Jayka (9)
|
MRFLY |
54.67 |
59.04 |
4.37 |
| 14 |
Wiygul, Clementina L (9)
|
MRFLY |
|
1:00.37 |
- |
| 15 |
Delinois, Ruby (9)
|
MRRED |
|
1:08.04 |
- |
| 16 |
Colbert, Sage (7)
|
MRRED |
|
1:08.77 |
- |
| 17 |
Zhong, Gloria (6)
|
MRFLY |
|
1:12.75 |
- |
|
Collins-Shurland, Isabella S (10)
|
MRCIY |
1:28.97 |
DQ |
- |