| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McAvinue, Kylie R (10)
|
MRYBAR |
39.04 |
39.04 |
0.00 |
| 2 |
Ryan, Addison (10)
|
MRWSSC |
39.04 |
39.58 |
0.54 |
| 3 |
Galvis Betancur, Jiolin (10)
|
MRYBAR |
44.06 |
44.21 |
0.15 |
| 4 |
Cassel, Juliette L (10)
|
MRPBAC |
46.56 |
45.57 |
-0.99 |
| 5 |
Odell, Eleanor V (10)
|
CTSYS |
46.48 |
46.48 |
0.00 |
| 6 |
Howe, June E (10)
|
MRNDAC |
48.15 |
49.22 |
1.07 |
| 7 |
Hayes-Hodge, Laray L (10)
|
MRWSSC |
52.55 |
51.40 |
-1.15 |
| 8 |
Posadas, Emilia M (9)
|
MRPBAC |
58.24 |
53.80 |
-4.44 |
| 9 |
Kraft, Braeyelle M (10)
|
MRNDAC |
54.59 |
54.44 |
-0.15 |
| 10 |
Rink, Hanna (10)
|
CTSYS |
52.70 |
56.05 |
3.35 |
| 11 |
Cardone, Caylee G (9)
|
MRPBAC |
|
56.70 |
- |
| 12 |
Bonnar, Emma E (9)
|
MRMWDA |
|
58.17 |
- |
| 13 |
Dowdy, Emma (10)
|
MRVAC |
59.88 |
1:00.56 |
0.68 |
| 14 |
Mohamed, Kenzie (10)
|
CTSYS |
1:07.23 |
1:01.62 |
-5.61 |
| 15 |
Barsalou, Serelle F (9)
|
CTSYS |
1:14.06 |
1:06.66 |
-7.40 |
| 16 |
Stent, Gabriella R (10)
|
MRMWDA |
|
1:11.29 |
- |
|
Santos, Angelina (9)
|
MRVAC |
1:25.19 |
DQ |
- |
|
Rowe, Gianna N (9)
|
MRPBAC |
1:15.81 |
DQ |
- |