| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Molina, Isabella E (13)
|
MRLIE |
26.52 |
26.36 |
-0.16 |
| 2 |
Sherman, Alexa L (13)
|
MRLIE |
26.85 |
26.70 |
-0.15 |
| 3 |
Spencer-Edwards, Lauren A (13)
|
MRQNS |
26.66 |
26.74 |
0.08 |
| 4 |
Karczewski, Cavan (13)
|
MRBAD |
27.66 |
26.78 |
-0.88 |
| 5 |
Curinnaia, Elena (13)
|
MRMPNY |
26.59 |
26.94 |
0.35 |
| 6 |
Gourevitch, Olivia (13)
|
MRWEST |
27.08 |
27.02 |
-0.06 |
| 7 |
Otto, Olivia L (13)
|
MRWEST |
27.11 |
27.03 |
-0.08 |
| 8 |
McGlynn, Clare E (13)
|
MRBAD |
26.86 |
27.15 |
0.29 |
| 9 |
Canales Alvizuri, Isabella N (13)
|
MRLIE |
27.20 |
27.17 |
-0.03 |
| 10 |
Khamutaeva, Ariuna (13)
|
MRMPNY |
27.80 |
27.77 |
-0.03 |
| 11 |
Jia, Nicole X (13)
|
MRMPNY |
26.79 |
27.89 |
1.10 |
| 12 |
Epps, Emani N (13)
|
MRBKR |
27.86 |
27.97 |
0.11 |
| 13 |
Diab, Ella G (13)
|
MRCOND |
28.32 |
28.29 |
-0.03 |
| 14 |
Pietruszka, Amelia S (13)
|
MRQNS |
28.16 |
28.46 |
0.30 |
| 15 |
Tse, Avery (13)
|
MRMAKO |
28.35 |
28.49 |
0.14 |
| 16 |
Leal, Jazmine (13)
|
MRRED |
28.72 |
28.56 |
-0.16 |
|
Suharev, Anna M (13)
|
MRFREE |
26.56 |
DQ |
- |
|
DiStefano, Ella (12)
|
MRTRI |
29.06 |
DQ |
- |