| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Best, Clark J (9)
|
MDCAA |
45.25 |
43.40 |
-1.85 |
| 2 |
Chen, Kevin Z (10)
|
MDEST |
45.93 |
44.09 |
-1.84 |
| 3 |
Chan, Max X (10)
|
MDCAA |
48.49 |
46.40 |
-2.09 |
| 4 |
Chiodo, Bennett J (9)
|
MDMAS |
55.12 |
47.33 |
-7.79 |
| 5 |
McGrain, Kieran (9)
|
MDCGA |
49.32 |
47.37 |
-1.95 |
| 6 |
Cohen, Jonathan M (10)
|
MDJCC |
47.63 |
47.41 |
-0.22 |
| 7 |
Trammell, Garrett (9)
|
MDBAY |
50.96 |
47.43 |
-3.53 |
| 8 |
Hoover, Declan B (9)
|
MDROCK |
51.06 |
48.06 |
-3.00 |
| 9 |
Simons, Owen (10)
|
MDMAS |
45.85 |
48.67 |
2.82 |
| 10 |
Gurvits, Dylan T (9)
|
MDMAS |
1:00.61 |
48.73 |
-11.88 |
| 11 |
Prachanronarong-Budo, Ryker (9)
|
MDJCC |
49.83 |
48.78 |
-1.05 |
| 12 |
Matthews, Jackson T (10)
|
MDGBSA |
51.52 |
48.79 |
-2.73 |
| 13 |
Cashion, Charles D (10)
|
MDASC |
59.50 |
49.79 |
-9.71 |
| 14 |
Sheremet, Kirill (9)
|
MDMAS |
53.00 |
51.06 |
-1.94 |
| 15 |
Gao, Jayden H (9)
|
MDCAA |
55.29 |
51.08 |
-4.21 |
| 16 |
Walker, Jackson R (9)
|
MDNBAC |
53.31 |
51.58 |
-1.73 |
| 17 |
Small, Parker M (9)
|
MDNBAC |
48.75 |
52.65 |
3.90 |
| 18 |
Kule-Thomas, Chukumalobi A (10)
|
MDRAC |
1:02.02 |
53.54 |
-8.48 |
| 19 |
Nguyen, William M (9)
|
MDMAC |
1:16.33 |
57.82 |
-18.51 |
| 20 |
Bird, Logan R (9)
|
MDFSC |
1:07.85 |
59.35 |
-8.50 |
| 21 |
House, Grant I (9)
|
MDMAC |
1:09.42 |
1:06.00 |
-3.42 |
| 22 |
Segovia, Jacob R (9)
|
MDFAST |
1:13.56 |
1:08.12 |
-5.44 |
| 23 |
Quintyne, Liam (9)
|
MDBAY |
1:12.93 |
1:13.47 |
0.54 |
| 24 |
Carter, Isaiah (10)
|
MDBAY |
1:36.41 |
1:15.37 |
-21.04 |
|
Stassi, Paul Z (10)
|
MDCAA |
43.73 |
DQ |
- |
|
Banaja, Zane (10)
|
MDBAY |
1:10.23 |
DQ |
- |
|
Zhang, Bowen (10)
|
MDRAC |
48.03 |
NS |
- |