| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilkerson, Clarke G (8)
|
PVNCAP |
41.06 |
38.22 |
-2.84 |
| 2 |
Lopez, Chelsea E (6)
|
PVTIBU |
40.07 |
42.86 |
2.79 |
| 3 |
Lei, Catherine N (7)
|
PVSA |
47.43 |
43.58 |
-3.85 |
| 4 |
Jaczko, Penrose M (8)
|
PVNCAP |
46.67 |
44.69 |
-1.98 |
| 5 |
Bustios, Isabella (8)
|
PVYSS |
50.22 |
47.21 |
-3.01 |
| 6 |
Berg, Naomi (8)
|
PVYSS |
45.09 |
48.58 |
3.49 |
| 7 |
Marroquin, Novaleigh M (7)
|
PVPAC |
55.59 |
48.79 |
-6.80 |
| 8 |
Fouzi, Nadia D (8)
|
PVERSC |
55.91 |
49.75 |
-6.16 |
| 9 |
Velazquez, Penelope T (6)
|
PVPAC |
52.19 |
50.07 |
-2.12 |
| 10 |
Cope, Zara (7)
|
PVYSS |
52.23 |
53.34 |
1.11 |
| 11 |
Tarr, Aria A (8)
|
PVPAC |
1:02.92 |
53.47 |
-9.45 |
| 12 |
Bernal, Jazlynn L (7)
|
PVPAC |
58.76 |
56.78 |
-1.98 |
| 13 |
Kaur, Rida (8)
|
PVNCAP |
|
59.48 |
- |
| 14 |
Ananfack, Julie B (8)
|
PVPAC |
53.66 |
1:00.15 |
6.49 |
| 15 |
Teles, Sylvia (8)
|
PVYSS |
|
1:01.28 |
- |
| 16 |
Indrakanti, Vibha S (7)
|
PVTIBU |
|
1:01.51 |
- |