| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Masneri, Luke M (10)
|
NCGCY |
2:26.57 |
2:18.48 |
-8.09 |
| 2 |
Galloway, Chase M (9)
|
NCTYDE |
2:20.82 |
2:20.41 |
-0.41 |
| 3 |
Morrison, McCulloch D (10)
|
VALY |
2:28.62 |
2:29.96 |
1.34 |
| 4 |
Hennis, Colin J (9)
|
NCTYDE |
2:30.64 |
2:31.38 |
0.74 |
| 5 |
Clay, Isaac S (10)
|
SCFAY |
2:33.27 |
2:33.93 |
0.66 |
| 6 |
Parker, Holden A (10)
|
NCTYDE |
2:36.99 |
2:34.88 |
-2.11 |
| 7 |
Zarzar, Daniel P (10)
|
NCYOTA |
2:45.20 |
2:35.58 |
-9.62 |
| 9 |
Lowther, Ian B (10)
|
NCYOTA |
2:32.40 |
2:37.43 |
5.03 |
| 10 |
Wu, Titus S (8)
|
VALY |
2:43.49 |
2:41.22 |
-2.27 |
| 11 |
Holland, Beckett A (10)
|
SCAAYM |
2:49.63 |
2:44.03 |
-5.60 |
| 12 |
Rau, Nicholas E (10)
|
VAVABR |
2:46.91 |
2:45.02 |
-1.89 |
| 13 |
Huffman, Remington M (10)
|
VAVABR |
2:51.80 |
2:45.53 |
-6.27 |
| 14 |
Barber, Brayden D (10)
|
NCYBAC |
2:42.40 |
2:45.94 |
3.54 |
| 15 |
Adekoya, Olamikun O (9)
|
NCGCY |
2:48.52 |
2:46.59 |
-1.93 |
| 16 |
Scott, Graham R (10)
|
NCWNCY |
2:45.32 |
2:46.69 |
1.37 |
| 17 |
Parnell, Cole (10)
|
NCYOTA |
2:48.60 |
2:47.44 |
-1.16 |
| 18 |
Leger, Benjamin D (8)
|
VALY |
2:49.68 |
2:50.19 |
0.51 |
| 19 |
Leon III, Nestor (10)
|
NCYBAC |
2:50.16 |
2:54.26 |
4.10 |
| 22 |
Vega Palomino, Xander (10)
|
NCFAST |
3:02.87 |
3:03.17 |
0.30 |