| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barlament, Ella C (10)
|
NCLOY |
38.34 |
39.04 |
0.70 |
| 2 |
Morgan, Anna B (10)
|
NCRMY |
41.50 |
41.20 |
-0.30 |
| 3 |
Supanich, Isabella J (10)
|
VAVABR |
41.88 |
41.30 |
-0.58 |
| 4 |
Grohman, Anneliese K (9)
|
NCTYDE |
42.23 |
41.99 |
-0.24 |
| 5 |
Willis, Hannah J (10)
|
NCGCY |
41.80 |
42.13 |
0.33 |
| 6 |
Heit, Annie B (10)
|
NCYOTA |
44.19 |
42.16 |
-2.03 |
| 7 |
Parish, Selah S (10)
|
VALY |
41.29 |
42.70 |
1.41 |
| 8 |
Donahue, Latham E (10)
|
NCWNCY |
46.56 |
45.31 |
-1.25 |
| 9 |
Cope, Violet E (10)
|
NCLOY |
46.32 |
45.35 |
-0.97 |
| 11 |
Bull, Ivy C (10)
|
NCWNCY |
50.94 |
45.90 |
-5.04 |
| 13 |
Tucker, Grace L (10)
|
NCTYDE |
46.27 |
47.06 |
0.79 |
| 15 |
Millsaps, Caroline M (10)
|
NCYSST |
45.83 |
47.81 |
1.98 |
| 16 |
Hewett, Anna E (8)
|
SCSYS |
52.77 |
48.20 |
-4.57 |
| 17 |
Eakkhattiyakorn, Tamara (10)
|
VALY |
48.52 |
48.82 |
0.30 |
| 18 |
Insixiengmay, Kelly P (10)
|
NCTYDE |
46.21 |
48.96 |
2.75 |
| 19 |
McConnell, Ayla (9)
|
NCTYDE |
47.89 |
49.03 |
1.14 |