| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schneider, Alana B (12)
|
NCYOTA |
5:35.98 |
5:40.01 |
4.03 |
| 2 |
Ross, Sadie B (12)
|
NCGCY |
5:46.47 |
5:43.59 |
-2.88 |
| 3 |
Donahue, Catherine E (12)
|
NCWNCY |
6:12.12 |
5:48.99 |
-23.13 |
| 4 |
Kloeck, Sarah L (12)
|
SCAAYM |
5:52.53 |
5:53.79 |
1.26 |
| 5 |
Pennington, Elaina M (11)
|
VALY |
6:11.80 |
5:55.68 |
-16.12 |
| 6 |
Cinson, Evelyn S (12)
|
NCYSST |
5:58.62 |
6:01.43 |
2.81 |
| 7 |
McKever, Hailey K (11)
|
SCAAYM |
6:35.53 |
6:01.93 |
-33.60 |
| 8 |
Jones, Scarlett (12)
|
NCYOTA |
6:02.62 |
6:02.15 |
-0.47 |
| 9 |
Smith, Molly E (12)
|
NCGCY |
6:14.52 |
6:10.05 |
-4.47 |
| 10 |
Peters, Milah N (12)
|
NCTRY |
6:13.23 |
6:10.73 |
-2.50 |
| 11 |
Smith, Avery T (12)
|
NCGCY |
6:31.98 |
6:16.73 |
-15.25 |
| 12 |
Hutchins, Landry C (11)
|
NCTYDE |
6:29.04 |
6:18.51 |
-10.53 |
| 13 |
Blazer, Callie A (12)
|
NCYOTA |
6:14.76 |
6:19.68 |
4.92 |
| 14 |
Mielke, Zoe I (12)
|
VAYOBX |
6:28.92 |
6:20.45 |
-8.47 |
| 15 |
McMillan, Charlotte H (11)
|
NCYOTA |
6:29.63 |
6:20.92 |
-8.71 |
| 16 |
Hinde, Natalie A (12)
|
NCYOTA |
6:34.96 |
6:35.78 |
0.82 |
| 17 |
Eiban, Emerson N (12)
|
VALY |
6:49.02 |
6:37.13 |
-11.89 |
| 18 |
Kasa, Keira L (11)
|
NCGCY |
6:47.68 |
6:47.52 |
-0.16 |