| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baker, Shelby L (11)
|
NIMERC |
42.40 |
43.15 |
0.75 |
| 2 |
Sanson, Charlotte E (11)
|
NIPMSC |
42.37 |
43.34 |
0.97 |
| 3 |
Lamb, Emily A (11)
|
NIMERC |
52.72 |
48.65 |
-4.07 |
| 4 |
Bugbee, Kyla C (11)
|
NIPMSC |
|
51.74 |
- |
| 5 |
Stonehouse, Sierra L (11)
|
NIGRSC |
|
53.84 |
- |
| 6 |
Kazmark, Raelyn J (11)
|
NIVICT |
1:04.43 |
55.66 |
-8.77 |
| 7 |
Trapp, Angelina L (11)
|
NIPMSC |
|
57.57 |
- |
| 8 |
Bartholomay, Meghan E (11)
|
NIGCOM |
1:11.85 |
1:00.12 |
-11.73 |
| 9 |
Campbell, Brooklyn M (11)
|
NIVICT |
1:07.29 |
1:03.73 |
-3.56 |
| 10 |
Walton, Ella G (11)
|
NIPMSC |
|
1:16.97 |
- |
|
Werner, Riley K (11)
|
NIVICT |
|
NS |
- |