| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hahn, Elizabeth P (13)
|
NISDSC |
26.80 |
26.95 |
0.15 |
| 2 |
Regelsberger, Emily C (14)
|
NIUN-V |
26.72 |
27.08 |
0.36 |
| 3 |
Ferrie, Elora M (14)
|
NIHAWK |
27.63 |
28.70 |
1.07 |
| 4 |
Taylor, Katie E (14)
|
NICAQY |
27.22 |
28.77 |
1.55 |
| 5 |
Bromage, Charlotte C (13)
|
NIUN |
28.70 |
29.29 |
0.59 |
| 6 |
Bryce, Emma L (14)
|
NIRRST |
28.66 |
29.30 |
0.64 |
| 7 |
Federico, Sydney A (14)
|
NIGRSC |
29.12 |
29.43 |
0.31 |
| 8 |
Solodova, Tatiana (13)
|
NIGCOM |
30.75 |
29.85 |
-0.90 |
| 9 |
Richards, Amelia L (13)
|
NIGRSC |
30.18 |
30.28 |
0.10 |
| 10 |
Brennan, Sarah D (14)
|
NIGRSC |
30.03 |
30.92 |
0.89 |
| 11 |
Geoca, Zoey L (14)
|
NISDSC |
32.24 |
31.13 |
-1.11 |
| 12 |
Reilly, Katie R (13)
|
NIHAWK |
33.27 |
31.69 |
-1.58 |
| 13 |
Hotaling, Asha G (13)
|
NIUN |
31.36 |
31.76 |
0.40 |
| 14 |
Melozzi, Layla I (13)
|
NIHAWK |
33.75 |
32.35 |
-1.40 |
| 15 |
Farnes, Alexi R (14)
|
NICAQY |
30.88 |
32.58 |
1.70 |
| 16 |
Gonnoud, Elizabeth B (13)
|
NIGRSC |
33.95 |
32.68 |
-1.27 |
| 17 |
Burns, Hannah B (13)
|
NIGRSC |
|
32.70 |
- |
| 18 |
Sponable, Julia E (13)
|
NIGRAT |
48.11 |
45.58 |
-2.53 |
|
Granger, Isabel C (14)
|
NIMERC |
28.18 |
NS |
- |
|
Nellis, Lillian M (13)
|
NIHAWK |
31.48 |
NS |
- |