| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Geary, Kaitlyn J (10)
|
NIMERC |
45.23 |
39.34 |
-5.89 |
| 2 |
Kelley, Michaela G (10)
|
NIUN |
47.00 |
45.56 |
-1.44 |
| 3 |
Santmyer, Grace E (10)
|
NISDSC |
48.66 |
45.85 |
-2.81 |
| 4 |
Siciliano, Paige E (10)
|
NIGRSC |
46.90 |
45.87 |
-1.03 |
| 5 |
Kazmark, Olivia N (10)
|
NIVICT |
47.33 |
46.37 |
-0.96 |
| 6 |
Smith, Lindsey A (10)
|
NIGRSC |
46.22 |
46.62 |
0.40 |
| 7 |
Beutner, Ella R (10)
|
NIUN |
48.00 |
47.64 |
-0.36 |
| 8 |
Schwandt, Natalie K (9)
|
NIGRAT |
50.10 |
48.20 |
-1.90 |
| 9 |
Young, Jazmine J (10)
|
NIGRSC |
|
50.15 |
- |
| 10 |
Kapral, Chloe H (10)
|
NIHAWK |
55.69 |
50.35 |
-5.34 |
| 11 |
Nguessamble, Tristyn D (10)
|
NISDSC |
47.74 |
50.68 |
2.94 |
| 12 |
Olmstead, Hannah E (9)
|
NIVICT |
56.28 |
51.13 |
-5.15 |
| 13 |
Olbrys, Lilja (10)
|
NIVICT |
51.90 |
51.19 |
-0.71 |
| 14 |
Strausser, Cadence A (9)
|
NIGRAT |
58.74 |
51.36 |
-7.38 |
| 15 |
Wagner, Keaghan J (9)
|
NISDSC |
52.49 |
51.46 |
-1.03 |
| 16 |
Chen, Chloe Y (9)
|
NISDSC |
56.68 |
51.69 |
-4.99 |
| 17 |
Richards, Kylie G (10)
|
NIHAWK |
|
51.70 |
- |
| 18 |
Crisco, Jacqueline E (9)
|
NIHAWK |
|
54.40 |
- |
| 19 |
Robinson, Sara C (10)
|
NISDSC |
53.73 |
54.54 |
0.81 |
| 20 |
Koneski Collette, Eliana J (10)
|
NIGRSC |
|
57.15 |
- |
| 21 |
Munson, Sophie M (9)
|
NIGRSC |
1:08.42 |
57.72 |
-10.70 |
| 22 |
Farley, Megan K (9)
|
NIGRSC |
|
57.90 |
- |
| 23 |
Kenny, Elisabeth A (10)
|
NIUN |
1:01.53 |
58.07 |
-3.46 |
| 24 |
Brockman, Reese S (10)
|
NIRRST |
|
1:01.16 |
- |
| 25 |
Rioux, Alexis E (9)
|
NIUN |
56.51 |
1:01.27 |
4.76 |
| 26 |
Bub, Emily R (9)
|
NIGCOM |
59.23 |
1:03.50 |
4.27 |
|
Gucker, Margaret A (10)
|
NIRRST |
|
NS |
- |
|
Stoos, Arianna E (10)
|
NIGRAT |
52.22 |
NS |
- |